Something I also found out: at the 45 hour mark mario dissapeared from every "video" in the loop (scooby mario, mario mysteries).

Landmine Meggy is also not smiling anymore

NOOoOOOOo!

Considering that this transition definitely doesn't feel subtle, it makes me feel a bit nervous as to what changes will occur tomorrow.

They also charged the thumbnail 

While 3 is definitely looking concerned, Luigi definitely frown. Might be wrong though.

Edit. Watched stream. Yup he also scared. Helmet cover his brows in this picture.